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Valiant Co Ltd taq polymerase buffer 10× mgcl2
Taq Polymerase Buffer 10× Mgcl2, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/taq+core+kit+10/Taq+CORE+Kit+(with+(NH4)2+SO4+buffer)/pm39082812-279-13-21
Average 96 stars, based on 38 article reviews
taq polymerase buffer 10× mgcl2 - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Rapid and selective detection of macrocyclic trichothecene producing Stachybotrys chartarum strains by loop-mediated isothermal amplification (LAMP)
Article Snippet: DNA concentrations were monitored using a NanoDrop ND1000 spectrophotometer (Peqlab Biotechnologie, Erlangen, Germany) according to the manufacturer’s recommendations. .. Chemotype-specific triplex PCR with DNA of S. chartarum isolates was performed using the Taq Core Kit 10 (MP Biomedicals) with the primers and cycling protocol described in [ ]. .. The PCR products were separated on 1.3% agarose gel (Biozym Scientific, Hessisch Oldendorf, Germany) at 120 V and 200 mAmp for 1 h and subsequently stained in a dimidium bromide bath for 10 min. Gels were subsequently washed with deionized water for 10 min before visual analysis on a UV transilluminator (UVT-28 M, Herolab, Wiesloch, Germany).

Article Title: Rapid and selective detection of macrocyclic trichothecene producing Stachybotrys chartarum strains by loop-mediated isothermal amplification (LAMP)
Article Snippet: DNA concentrations were monitored using a NanoDrop ND1000 spectrophotometer (Peqlab Biotechnologie, Erlangen, Germany) according to the manufacturer’s recommendations. .. Chemotype-specific triplex PCR with DNA of S. chartarum isolates was performed using the Taq Core Kit 10 (MP Biomedicals) with the primers and cycling protocol described in [34]. .. The PCR products were separated on 1.3% agarose gel (Biozym Scientific, Hessisch Oldendorf, Germany) at 120 V and 200 mAmp for 1 h and subsequently stained in a dimidium bromide bath for 10 min. Gels were subsequently washed with deionized water for 10min before visual analysis on a UV transilluminator (UVT-28 M, Herolab, Wiesloch, Germany).

Article Title: A new model for fatty acid hydroxylase-associated neurodegeneration reveals mitochondrial and autophagy abnormalities
Article Snippet: The cDNA was inserted into the overexpression vector “pUAST-attB” ( ) using the “NEBuilder Hifi DNA assembly cloning kit” following the manufacturer’s protocol. .. To generate cDNA with overhangs complementary to the vector, a PCR was performed using the Taq CORE Kit 10 from MP Biomedicals (11EPTQK101-CF) and the HiFi primers listed in . .. The vector was linearized using the restriction enzymes KpnI-HF (R3142) and EagI-HF (R3505) from New England Biolabs according to the manufacturer’s protocol by incubation for 1 h at 37°C.

Plasmid Preparation:

Article Title: A new model for fatty acid hydroxylase-associated neurodegeneration reveals mitochondrial and autophagy abnormalities
Article Snippet: The cDNA was inserted into the overexpression vector “pUAST-attB” ( ) using the “NEBuilder Hifi DNA assembly cloning kit” following the manufacturer’s protocol. .. To generate cDNA with overhangs complementary to the vector, a PCR was performed using the Taq CORE Kit 10 from MP Biomedicals (11EPTQK101-CF) and the HiFi primers listed in . .. The vector was linearized using the restriction enzymes KpnI-HF (R3142) and EagI-HF (R3505) from New England Biolabs according to the manufacturer’s protocol by incubation for 1 h at 37°C.

Amplification:

Article Title: Polyphasic characterization of Aspergillus section Flavi isolated from animal feeds in Algeria
Article Snippet: .. Amplification (for both CaM gene and ITS region) was set up following manufacturer's instructions in a total volume of 25 μl using a Taq Core kit 10 (MP Biomedicals). .. The PCR reaction involved a denaturation cycle at 94 C for 4 min, followed by 30 cycles of a series of denaturation step at 94 C for 45 s, annealing for 45 s at 55 C for the ITS region and 57 C for the CaM gene, and extension at 72 C for 50 s, followed by a final extension step at 72 C for 10 min. PCR products were checked by electrophoresis on agarose gel after staining with GelRedTM Nucleic Acid Gel Stain (Biotium).



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